Digital scale-up and optimization of animal cell cultures
Cell culture engineering principles. Kinetic analysis. Knowledge simulation models. Digital optimization of batch, fed-batch and perfusion culture bioreactors
ONLINE COURSE: On-demand
COURSE FEE: 350 € per session
COURSE ORGANIZATION
Course organized in 7 sessions
Sessions can be taken individually
Scheduling: suggested one session per week
Effort: 3 - 6 hours per session
COURSE DESCRIPTION
The course provides a comprehensive overview of the concepts and methodologies of bioprocess engineering for the digital design and optimization of animal cell culture bioreactors. It covers cell culture kinetics, oxygen transfer in bioreactors, the development of knowledge-driven simulation models, the scale-up and optimal operations of batch, fed-batch and continuous perfusion bioreactors. The course combines methodological review presentations with an industrial case study on monoclonal antibodies production.
INSTRUCTOR
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Jean-Marc Engasser, BioProcess Digital
DIGITAL LEARNING
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Learning platform with course resources
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Live or recorded slideshow presentations
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Case studies on spreadsheet templates with guides and corrections
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Online collective or one-to-one tutoring
COURSE PROGRAM
SESSION 1: CELL CULTURE ENGINEERING PRINCIPLES
Animal cells kinetics. Mass transfer in bioreactors. Mass balances in bioreactors
SESSION 2: CELL CULTURE KINETICS
Kinetic analysis in batch and continuous cultures. Determination of culture and cellular rates. Kinetic laws
SESSION 3: OXYGEN TRANSFER AND CONSUMPTION KINETICS
Kinetic analysis of oxygen transfer and cellular uptake. Oxygen solubility and transfer rate laws
SESSION 4: CELL CULTURE BIOREACTOR SIMULATION MODEL
Influencing biochemical and physical phenomena. Simulation model relations. Numerical integration on spreadsheet.
SESSION 5: BATCH CULTURE SIMULATION AND OPTIMIZATION
Batch bioreactor principles and operational variables. Batch culture simulation and optimal operation
SESSION 6: FED_BATCH CULTURE SIMULATION AND OPTIMIZATION
Fed-batch bioreactor principles and operational variables. Fed-batch culture simulation and optimal operation
SESSION 7: PERFUSION CULTURE SIMULATION AND OPTIMIZATION
Perfusion bioreactor principles and operational variables. Perfusion culture simulation and optimal operation